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Laboratory Tools

Peptide
Reconstitution

Calculate solvent volumes for accurate in-vitro stock solution preparation. For qualified laboratory and research use only.

⚠️ Research Use Only — This calculator is intended solely for laboratory researchers preparing peptide stock solutions for in-vitro experiments. It does not constitute dosing guidance of any kind. All calculations are for scientific research applications only.
Reconstitution Calculator
Determine the volume of solvent (bacteriostatic water or acetic acid) required to achieve a target stock solution concentration from a lyophilised peptide.
LYOPHILISED PEPTIDE — mg
mg/mL
Solvent Volume
—
mL
Stock Concentration
—
mg/mL
μg per μL
—
μg/μL
Formula Applied
Volume (mL) = Mass (mg) ÷ Target Concentration (mg/mL)
μg/μL = Concentration (mg/mL) × 1

Aliquot and store immediately after reconstitution. Avoid repeated freeze-thaw cycles.
Working Concentration Calculator
Calculate the required dilution of your stock solution to achieve a target working concentration for in-vitro assays.
Stock Volume Needed
—
μL
Diluent to Add
—
μL
Dilution Factor
—
×
Serial Dilution Calculator
Plan serial dilution series for dose-response curves and in-vitro assays.
Step Concentration Stock (μL) Diluent (μL)

Solvent Selection Guide

  • Bacteriostatic Water — suitable for most hydrophilic peptides. Preferred for aqueous-soluble compounds.
  • Acetic Acid 0.6% — for poorly water-soluble or hydrophobic peptides (e.g. TB-500). Dilute further with PBS before use in assay.
  • DMSO — for highly lipophilic small molecule peptides. Max 0.1% DMSO in final assay to avoid cytotoxicity.
  • PBS pH 7.4 — physiological buffer, suitable for most cell culture applications.

Storage Best Practices

  • Store lyophilised peptides at -20°C in a desiccated environment
  • After reconstitution, aliquot immediately into single-use volumes
  • Avoid repeated freeze-thaw cycles — degrade potency
  • Label all aliquots with: compound, concentration, date, batch
  • Reconstituted solutions: stable 2-4 weeks at -20°C, 1-2 weeks at 4°C
  • Some peptides (e.g. GHK-Cu) are light-sensitive — use amber vials
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